Once we receive your sample, we will process it as follows:
- Firstly, we will extract DNA from your infected leaf sample. This will include DNA from the wheat as well as any microorganisms residing on the leaf.
- We will then use a molecular biology assay to probe the DNA for presence of the Yellow Spot pathogen.
- If we identify Yellow Spot, we will then place your leaf sample on a rich media to allow the fungal pathogen to grow out.
- Once the fungal hyphae have emerged, we will then induce the fungus to sporulate. We will transfer a single spore (conidium) on to fresh media to select a pure fungal strain.
- Once the fungus has grown, we will extract the DNA and test for genes encoding effectors (host-selective toxins). This will include genes encoding effectors from overseas which are biosecurity risks to Australia.
All of this information will enable us to build a snapshot of the status of Yellow Spot within Australia. Yellow spot strains from resistant wheat varieties are high priorities for pathogenicity assays (plant infections) to look for any shifts in virulence. It is crucial to recover current strains of the fungus, monitor the pathogen and stay on top of any changes, so we are best placed to combat it.